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Chromatin Immunoprecipitation of TRA-1 in C. elegans and C. briggsae

ABSTRACT: The goal of this study is to identify and characterize sites in the C. elegans genome bound by the transcription factor TRA-1. TRA-1 ChIP-seq was performed in the following stages of animals in duplicate: 1) L2 stage of C. elegans wild-type N2 strain; 2) L3 stage of C. elegans wild-type N2 strain; 3) young adult stage of C. elegans glp-4(bn2) mutant; 4) young adult stage of C. elegans spe-11(hc77) mutant; 5) L3 stage of C. briggsae wild-type AF16 strain. As a negative control, TRA-1 ChIP-seq was also performed in C. elegans L3 stage with tra-1(e1834) homozygous and heterozygous mutation. Input DNA was also sequenced in each condition.

ORGANISM(S): Caenorhabditis briggsae

SUBMITTER: Kohta Ikegami

PROVIDER: E-GEOD-48917 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TRA-1 ChIP-seq reveals regulators of sexual differentiation and multilevel feedback in nematode sex determination.

Berkseth Matt M Ikegami Kohta K Arur Swathi S Lieb Jason D JD Zarkower David D

Proceedings of the National Academy of Sciences of the United States of America 20130917 40

How sexual regulators translate global sexual fate into appropriate local sexual differentiation events is perhaps the least understood aspect of sexual development. Here we have used ChIP followed by deep sequencing (ChIP-seq) to identify direct targets of the nematode global sexual regulator Transformer 1 (TRA-1), a transcription factor acting at the interface between organism-wide and cell-specific sexual regulation to control all sex-specific somatic differentiation events. We identified 184 ...[more]

PMID: 24046365

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