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Functional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (ribosome)

ABSTRACT: We present a basic characterization of the function of Y-box binding proteins in C. elegans. Besides playing an important role for fertility in the germline (all four CEY proteins), we found that the presence of CEY-1 and CEY-4 is essential for the assembly of larger polysomes in the soma. We therefore performed ribosome-profiling in combination with total RNA sequencing in wild type and cey-1,-4 double mutant animals to globally compare mRNA levels and their translation status. Ribosome-profiling analysis was peformed on wild type and cey-1,-4 mutant animals in duplicates. Four samples in total.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Dimos Gaidatzis 

PROVIDER: E-GEOD-62859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional characterization of C. elegans Y-box-binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes.

Arnold Andreas A   Rahman Md Masuder MM   Lee Man Chun MC   Muehlhaeusser Sandra S   Katic Iskra I   Gaidatzis Dimos D   Hess Daniel D   Scheckel Claudia C   Wright Jane E JE   Stetak Attila A   Boag Peter R PR   Ciosk Rafal R  

Nucleic acids research 20141105 21

The cold shock domain is one of the most highly conserved motifs between bacteria and higher eukaryotes. Y-box-binding proteins represent a subfamily of cold shock domain proteins with pleiotropic functions, ranging from transcription in the nucleus to translation in the cytoplasm. These proteins have been investigated in all major model organisms except Caenorhabditis elegans. In this study, we set out to fill this gap and present a functional characterization of CEYs, the C. elegans Y-box-bind  ...[more]

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