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LEM-2 ChIP-seq to study chromatin anchoring

ABSTRACT: Chromatin Immunoprecipitation against the nuclear envelope protein LEM-2 to detect association to the nuclear periphery. LEM-2 ChIP was performed on early embryo fixed extracts for wild-type and two mutants that have altered position of a perinuclear chromatin reporter. LEM-2 ChIP on early embryo chromatin extracts of three different genotypes: wild-type, met-2 set-25_delta_delta and cec-4_delta, in two independent biological replicas

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Dimos Gaidatzis

PROVIDER: E-GEOD-74133 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Perinuclear Anchoring of H3K9-Methylated Chromatin Stabilizes Induced Cell Fate in C. elegans Embryos.

Gonzalez-Sandoval Adriana A Towbin Benjamin D BD Kalck Veronique V Cabianca Daphne S DS Gaidatzis Dimos D Hauer Michael H MH Geng Liqing L Wang Li L Yang Teddy T Wang Xinghao X Zhao Kehao K Gasser Susan M SM

Cell 20151119 6

Interphase chromatin is organized in distinct nuclear sub-compartments, reflecting its degree of compaction and transcriptional status. In Caenorhabditis elegans embryos, H3K9 methylation is necessary to silence and to anchor repeat-rich heterochromatin at the nuclear periphery. In a screen for perinuclear anchors of heterochromatin, we identified a previously uncharacterized C. elegans chromodomain protein, CEC-4. CEC-4 binds preferentially mono-, di-, or tri-methylated H3K9 and localizes at th ...[more]

PMID: 26607792

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