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Optimized siRNAs with a minimal off-target effect facilitate the screening of essential genes in cancer cells

ABSTRACT: siRNAs with dN are a promising and easy approach to characterize essential genes by minimizing the off-target effect in cancer cells. mRNA profiles of HCT 116 cells after the transfection of siRNAs or blank control by deep sequencing, using HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Zhenzhen Liu 

PROVIDER: E-GEOD-62968 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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siRNAs with decreased off-target effect facilitate the identification of essential genes in cancer cells.

Li Chunyan C   Liu Zhenzhen Z   Yang Fang F   Liu Wensheng W   Wang Di D   Dong Encheng E   Wang Yu Y   Wu Chung-I CI   Lu Xuemei X  

Oncotarget 20150801 25

Since the essential genes are crucial to the proliferation and survival of cancer cells, the interference of these genes is promising to be an option for cancer therapy to overcome heterogeneity. However, the essential genes are highly overestimated by RNA interference (RNAi) screenings, which is mainly caused by the pervasive off-target effect of small interference RNA (siRNA) and short hairpin RNA (shRNA). In the present study, we designed Match-Mismatch paired siRNAs to discriminate the on-ta  ...[more]