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Analysis to evaluate the presence of 5’ cap in intragenic lncRNAs


ABSTRACT: RNA aliquots were digested with 5’-phosphate-dependent exonuclease (5'-exo) following pre-treatment with tobacco acid pyrophosphatase (TAP) to release the 5’ cap and render the RNA susceptible to 5’ exonuclease digestion (TAP+/5'-exo+). Two color array hybridizations were performed and TAP+/5'-exo+ samples were compared with TAP-/5'-exo+ control samples. Two-condition experiment, TAP+/5'-exo+ vs. TAP-/5'-exo+ treated total RNA samples. Biological replicates: 4 with dye swap

ORGANISM(S): Homo sapiens

SUBMITTER: Eduardo Reis 

PROVIDER: E-GEOD-62984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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