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Xenopus Piwi protein associated transcripts indicate regulation beyond transposons

ABSTRACT: This study examines the population of transcripts associated with the Xenopus Piwi proteins, Xiwi and Xili, from X.laevis and X.tropicalis. RIP-seq, CLIP-seq, piRNA-seq, and mRNA-seq datasets were integrated to determine how the Xenopus Piwi proteins where using piRNAs and binding interactions to associate with transcripts in gonadal cells. We performed several replicates of a Piw CLIP-Seq experiment to isolate RNA fragments as CLIP-tags to discover which transcripts are preferentially bound by the Piwi protein. Then we performed several types of mRNA expression profiling experiments using several forms of mRNA-Seq library construction formats. Finally, we sequenced the piRNAs from the OSS cells

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Nelson Lau

PROVIDER: E-GEOD-63228 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome and transposable element dynamics during PIWI regulation in Drosophila ovarian cell cultures

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