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Induction of ER stress in HCT116 colon cancer cells

ABSTRACT: To investigate the role of p53 and DICER in the induction of ER stress, wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells were treated with the ER stress inducers tunicamycin or brefeldin A for 24 hours. Microarray analysis was used to determine changes in gene expression associated with the induction of ER stress, and to compare this induction in wildtype, p53 knockout or DICER mutant HCT116 colon cancer cells Triplicate samples of HCT116 wildtype, HCT116 p53 knockout and HCT116 DICEREX5/EX5 cells were treated with with 0.5 mg/ml of BFA or 2 mg/ml of Tm for 24 h. Following treatment, cells were harvested and lysed in TRIzol reagent and RNA was extracted. Microarray analysis was carried out using Affymetrix HG-U133_Plus-2 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Sharon Glynn 

PROVIDER: E-GEOD-63252 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A close connection between the PERK and IRE arms of the UPR and the transcriptional regulation of autophagy.

Deegan Shane S   Koryga Izabela I   Glynn Sharon A SA   Gupta Sanjeev S   Gorman Adrienne M AM   Samali Afshin A  

Biochemical and biophysical research communications 20141202 1

Endoplasmic reticulum (ER) stress is known to lead to activation of both the unfolded protein response (UPR) and autophagy. Although regulatory connections have been identified between the UPR and autophagy, it is still unclear to what extent the UPR regulates the genes involved at the different stages of the autophagy pathway. Here, we carried out a microarray analysis of HCT116 cells subjected to ER stress and observed the transcriptional upregulation of a large cohort of autophagy-related gen  ...[more]

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