CKIT+ sorted cells from 57-137 day old fetal testes and ovaries
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Sofia Gkountela
PROVIDER: E-GEOD-63394 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
DNA Demethylation Dynamics in the Human Prenatal Germline.
Gkountela Sofia S Zhang Kelvin X KX Shafiq Tiasha A TA Liao Wen-Wei WW Hargan-Calvopiña Joseph J Chen Pao-Yang PY Clark Amander T AT
Cell 20150521 6
Global DNA demethylation in humans is a fundamental process that occurs in pre-implantation embryos and reversion to naive ground state pluripotent stem cells (PSCs). However, the extent of DNA methylation reprogramming in human germline cells is unknown. Here, we performed whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq) of human prenatal germline cells from 53 to 137 days of development. We discovered that the transcriptome and methylome of human germline is distinct from ...[more]
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Project description:We performed WGBS analyses on 6 human fetal samples at 53-137 days of development, 4 female and 2 male. We show that methylation reprogramming in the human germline is global yet incomplete with exons, 3’UTRs and human-specific transposons remaining methylated. Whole Genome Bisulfite-Seq of cKIT+ cells analyzed from 4 biological samples for fetal ovaries from 57-113 days of development and 2 samples for fetal testes at 59 and 137 days of development.
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