H3K4me1 ChIP-Seq experiment using cells from stage 15 Drosophila melanogaster embryos sorted by reporter gene activity
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ABSTRACT: The aim of this study was to analyze if transcriptionally active svb loci show enrichment for H3K4me1. To capture cells where svb-related ventral enhancers are active, we FACS-sorted nuclei from stage 15 Drosophila melanogaster embryos based on the expression of different reporter genes driven by different svb enhancers. The svb_E10 enhancer drives the expression of GFP, whilst dsRed is driven by the svb_7 enhancer. The sorted nuclei were then used for ChIP with anti-H3K4me1 and anti-H3 (for normalization purposes) antibodies.
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Drosophila melanogaster
SUBMITTER:
PROVIDER: E-MTAB-12396 | biostudies-arrayexpress |
SECONDARY ACCESSION(S): ERP142621
REPOSITORIES: biostudies-arrayexpress
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