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Comparison of CD8+ memory T-cells from fresh and high density precultured PBMC.

ABSTRACT: High density cultured CD8+ memory T-cells from peripheral blood mononuclear cells (PBMC) were found to be more sensitive towards antigenic stimulation. We used microarrays to detail the global programme of gene expression underlying differences in the cell density of human PBMC and identified genes that are significantly up- or downregulated dependent on the cell density of PBMC. Paired analysis of CD8+ memory T-cells from fresh and high density precultured PBMC from three healthy donors.

ORGANISM(S): Homo sapiens

SUBMITTER: Claus Scholz

PROVIDER: E-GEOD-63430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-density preculture of PBMCs restores defective sensitivity of circulating CD8 T cells to virus- and tumor-derived antigens.

Wegner Julia J Hackenberg Stephan S Scholz Claus-Jürgen CJ Chuvpilo Sergey S Tyrsin Dmitry D Matskevich Alexey A AA Grigoleit Götz Ulrich GU Stevanović Stefan S Hünig Thomas T

Blood 20150529 2

Peripheral blood mononuclear cells (PBMCs) are the only source of human lymphoid cells routinely available for immunomonitoring of T-cell responses to microbial and tumor-associated antigens. However, previous work in mice and humans had indicated that CD4 T cells transiently lose antigen sensitivity when cellular contacts are lost (eg, by entering the circulation). Using the simple and robust protocol for resetting T cells to original reactivity (RESTORE; ie, preculturing PBMCs for 2 days at a ...[more]

PMID: 26024876

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2015-06-09 | E-GEOD-51288 | biostudies-arrayexpress

Homo sapiens

Project description:Comparison of CD8+ memory T-cells from fresh and high density precultured PBMC.

| PRJNA267728 | ENA

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