Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of CD8+ memory T-cells from fresh and high density precultured PBMC.

ABSTRACT: High density cultured CD8+ memory T-cells from peripheral blood mononuclear cells (PBMC) were found to be more sensitive towards antigenic stimulation. We used microarrays to detail the global programme of gene expression underlying differences in the cell density of human PBMC and identified genes that are significantly up- or downregulated dependent on the cell density of PBMC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE63430 | GEO | 2015/06/09

SECONDARY ACCESSION(S): PRJNA267728

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Comparison of CD8+ memory T-cells from fresh and high density precultured PBMC.

Project description:High density cultured CD8+ memory T-cells from peripheral blood mononuclear cells (PBMC) were found to be more sensitive towards antigenic stimulation. We used microarrays to detail the global programme of gene expression underlying differences in the cell density of human PBMC and identified genes that are significantly up- or downregulated dependent on the cell density of PBMC. Paired analysis of CD8+ memory T-cells from fresh and high density precultured PBMC from three healthy donors.

2015-06-09 | E-GEOD-63430 | biostudies-arrayexpress

Expression data from human CD4 or CD8 T-cells isolated from PBMC cultured at a low cell density (LDC) or high cell density (HDC)

Project description:Research in human immunobiology is mainly based on working with peripheral blood mononuclear cells (PBMC). However, recent investigations have shown that circulating CD4+ T cells are less sensitive to several T-cell activating monoclonal antibodies (mAb) and to recall antigens as compared to tissue-resident cells or cells that were in-vitro cultured at a high cell density of 10^7 cells/mL for 2 days at 37°C and 5% CO2 (RESTORE protocol, Römer et al., Blood 2011, PMID: 21931118). To explain the increase in sensitivity of CD4+ T-cells to mAbs and recall antigens on a molecular level, we performed microarray hybridizations of total RNA from T-cells isolated from PBMC that were cultured at a low or high cell density. To avoid the detection of genes that are up- or down-regulated by the culture process itself, we used low cell density cultured PBMC, instead of freshly prepared PBMC. We used microarrays to detail the global programme of gene expression underlying differences in the cell density of human PBMC and identified genes that are significantly up- or downregulated dependent on the cell density of PBMC.

2015-06-09 | GSE51288 | GEO

Expression data from human CD4 or CD8 T-cells isolated from PBMC cultured at a low cell density (LDC) or high cell density (HDC)

2015-06-09 | E-GEOD-51288 | biostudies-arrayexpress

Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube and standard density gradient

2015-08-28 | GSE67321 | GEO

Gene expression changes in Peripheral Blood Mononuclear cells (PBMC) induced by physical activity

Project description:Gene expression changes in Peripheral Blood Mononuclear cells (PBMC) induced by physical activity was investigated in sedentary middle-aged men (mean age 52.6 years and BMI 29.1) who undertook a 24-week physical activity programme with blood sampling in the pre-exercise period , at the end of 24-weeks prescribed physical activity , and following a two-week detraining period. AgilentTM Whole Human Genome Oligo Microarrays were utilised to examine the effects of physical activity on mRNA expression profiles of the Peripheral Blood Mononuclear cells (PBMC) at 3 time points (pre-exercise, after 24 weeks physical activity, and at 26 weeks after 2 weeks detraining. There were 12 participants in this programme.

2010-08-03 | E-GEOD-12385 | biostudies-arrayexpress

Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube and standard density gradient

Project description:High quality genetic material is an essential pre-requisite when analyzing gene expression using microarray technology. Peripheral blood mononuclear cells (PBMC) are frequently used for genomic analyses, but several factors can affect the integrity of nucleic acids prior to their extraction, including the methods of PBMC collection and isolation. In this study, we compared the Ficoll-Paque density gradient centrifugation and BD Vacutainer cell preparation tube (CPT) protocols to determine if either method offered a distinct advantage in preparation of PBMC-derived immune cell subsets for their use in gene expression analysis. We compared gene expression in PBMC and individual immune cell types from Ficoll and CPT isolation protocols using Affymetrix microarrays. PBMC from 3 healthy donors were isolated using Ficoll-Paque gradient or BD Vacutainer CPT methods. Subsequently, immune cell subsets were separated by positive selection from PBMC obtained by both methods. RNA was extracted from total PBMC isolated by both protocols and from their subsets, followed by analysis of gene expression using Affymetrix microarrays. Gene expression was compared between Ficoll and CPT samples for a given cell type.

2015-08-28 | E-GEOD-67321 | biostudies-arrayexpress

Expression data from human subsets of PBMC

Project description:Human PBMC, there are major T, B, Dc, Basophil, Eosinophil and Neutrophil subsets with typical biological functions. The characteristic behavior of each subset was determined by its unique gene expression and regulation. Difference between each subset or different treatment to the same subset will induce huge amount different gene expression. This will provide the basic evidence of understanding why and how each subset cells functions properly. We used microarrays to detail the global programme of gene expression in each subsets of human PBMC and identified distinctly up-regulated genes during this process.

2018-01-19 | GSE109348 | GEO

Gene expression changes in Peripheral Blood Mononuclear cells (PBMC) induced by physical activity

2010-08-03 | GSE12385 | GEO

Altered porcine PBMC proteome and secretome after stimulation with BanLec

Project description:With this experiment, we aimed at showing changes in the proteome and secretome of porcine peripheral blood mononuclear cells (PBMC) after stimulation with Banana Lectin (BanLec).

2021-08-26 | PXD027505 | Pride

Juvenile Rheumatoid Arthritis Profiles In PBMC and SFMC

Project description:Biotinylated cRNA was synthesized from total RNA (Enzo; Farmingdale, NY) and processed according to the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix; Santa Clara, CA). 57 samples: 5 pauciarticular PBMC, 15 polyarticular PBMC, 11 control PMBC, 6 JSpA PBMC, 5 pauciarticular SFMC, 10 polyarticular PBMC, 5 JSpA SFMC classified by course. Keywords = juvenile rheumatoid arthritis (JRA) Keywords = peripheral blood mononuclear cells (PBMC) Keywords = synovial fluid mononuclear cells (SFMC) Keywords: other

2004-08-11 | GSE1402 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data