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MicroRNA targetome analysis during HCMV infection

ABSTRACT: To elucidate miRNA-mediated temporal crosstalk during productive infection, we identified genome-wide miRNA target sites using Argonaute-crosslinking and immunoprecipitation followed by high-throughput sequencing (AGO-CLIPseq) in human cytomegalovirus (HCMV)-infected cells and evaluated the targeting efficacy by applying our new AGO-CLIPseq enrichment (ACE)-scoring algorithm. To uncover the miRNA targetome in uninfected or infected human foreskin fibroblasts with HCMV (24, 48 and 72 post-infection hour) were subjected to take AGO-CLIPseq as well as mRNAseq/smallRNAseq.

ORGANISM(S): Homo sapiens

SUBMITTER: Daekwan Seo

PROVIDER: E-GEOD-63797 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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