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Genetic analysis reveals a longevity associated protein modulating endothelial function and angiogenesis

ABSTRACT: Transcriptome analysis in HEK293T transfected with plasmid carrying different isoforms of BPIFB4 gene. This gene was previously associated with exceptional longevity in a GWAS study performed on three different populations. Results indicate an up-regulation of stress response genes and proteostasis genes in HEK293T transfected with plasmid carrying the longevity-associated variant (LAV) of BPIFB4. Total RNA obtained from HEK293T over-expressing wild-type or mutated form of BPIFB4.

ORGANISM(S): Homo sapiens

SUBMITTER: Francesco Villa

PROVIDER: E-GEOD-63912 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic Analysis Reveals a Longevity-Associated Protein Modulating Endothelial Function and Angiogenesis.

Villa Francesco F Carrizzo Albino A Spinelli Chiara C CC Ferrario Anna A Malovini Alberto A Maciąg Anna A Damato Antonio A Auricchio Alberto A Spinetti Gaia G Sangalli Elena E Dang Zexu Z Madonna Michele M Ambrosio Mariateresa M Sitia Leopoldo L Bigini Paolo P Calì Gaetano G Schreiber Stefan S Perls Thomas T Fucile Sergio S Mulas Francesca F Nebel Almut A Bellazzi Riccardo R Madeddu Paolo P Vecchione Carmine C Puca Annibale A AA

Circulation research 20150601 4

<h4>Rationale</h4>Long living individuals show delay of aging, which is characterized by the progressive loss of cardiovascular homeostasis, along with reduced endothelial nitric oxide synthase activity, endothelial dysfunction, and impairment of tissue repair after ischemic injury.<h4>Objective</h4>Exploit genetic analysis of long living individuals to reveal master molecular regulators of physiological aging and new targets for treatment of cardiovascular disease.<h4>Methods and results</h4>We ...[more]

PMID: 26034043

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Genetic analysis reveals a longevity associated protein modulating endothelial function and angiogenesis

Project description:Transcriptome analysis in HEK293T transfected with plasmid carrying different isoforms of BPIFB4 gene. This gene was previously associated with exceptional longevity in a GWAS study performed on three different populations. Results indicate an up-regulation of stress response genes and proteostasis genes in HEK293T transfected with plasmid carrying the longevity-associated variant (LAV) of BPIFB4.

2015-06-12 | GSE63912 | GEO

Differential expression of genes after over expression of miR-23a~27a~24-2 cluster in human embryonic kidney cells

Project description:The experiment was deigned to identify the genes which get altered after the over expression of miR-23a~27a~24-2 cluster in HEK293T cells. The HEK293T cells plated in 6-well plate were transfected with two different concentrations of cloned miRNA cluster i.e 2micrograms and 4micrograms per well. Biological duplicates of 3 samples were used viz. control HEK293T cells, HEK293T cells transfected with 2micrograms of the cloned cluster and HEK293T cells transfected with 4micrograms of the cloned cluster.

2010-07-30 | E-GEOD-20158 | biostudies-arrayexpress

Gene expression changes in HEK293T cells upon overexpression of ING1b and GADD45a

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2013-02-04 | E-GEOD-43317 | biostudies-arrayexpress

The deafness gene DFNA5 induces programmed cell death through mitochondria and MAPK-related pathways [HEK293T]

Project description:The function of DFNA5 remained unknown for a long time, but previous functional studies by Op de Beeck et al. (2011) revealed that DFNA5 induces a growth defect in mutDFNA5- transfected HEK293T cells, as well as other cells, leading to PCD (Op de Beeck et al., 2011). The cell death-inducing capacity of DFNA5 was not only restricted to human cell lines, but was also observed in the yeast model Saccharomyces cerevisiae (Van Rossom et al., 2012). This inspired us to perform a transcriptomic analysis using two different model organisms (mammalian, HEK293T, and yeast, S.cerevisiae) to further elucidate the mechanisms related to DFNA5. Comparison of two HEK293T transfected cell populations, either with a WT or mutated DFNA5 gene. There are 5 biol reps for the WT transfected cells and 6 biol reps for the mut DFNA5 transfected cells.

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Inhibition of uPA expression by CRISPR-dCas9 DNA methyltransferases

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Imperfect centered miRNA binding sites are common and can mediate functional repression of target mRNAs.

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2021-09-09 | PXD021039 | Pride

overexpression of a newly truncated FMRP isoform with 297 amino acid

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Functional assessment of a new PBX1 variant through CRISPR-Cas9 gene editing

Project description:To assess the role of a variant of unknown significance in PBX1 (p.(Arg107Gln) by generating a HEK293T-derived cell line knockdown for PBX1 (clone 22 cells) through CRISPR-Cas9. We then transfected the KD cells or the WT HEK293T cells with a plasmid encoding either WT or Arg107Gln PBX1 proteins.

2023-02-28 | GSE215967 | GEO

Gene Expression Profile for the identification of miR-126 targets

Project description:"The analysis was designed to search for putative seed sequences for miR-126 in the 3M-bM-^@M-^YUTR of up-regulated genes. ECs were co-transfected with anti-miR-126 and a plasmid vector bearing the GFP-coding sequence and three complementary sites for miR-126 downstream. As a consequence of the presence of miR-126 binding sites the GFP expression was under the control of miR-126. Fluorescente cells were the cells transfected with anti-miR-126 oligo. MiR-126 target enrichment analysis was performed on the basis of the Miranda database, as provided by Diana miRGen. Significance was estimated on the basis of hyper geometric distribution p-values comparing the occurrence of miR targets in the signature with respect to the universe, defined as the genes expressed in HUVEC. Frequency of log 2 ratio between the subset of mir-126 targets and the genes which are not targets of miR-126 were plotted, highlighting enrichment of the target of miR in differentially regulated genes. " 3 x biological replicates of transiently trasfected cells with anti miR-126 oligo (LNA), and controls oligo. 2 biological replicates for GFP are included as technical controls.

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