Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from subsets of mouse spleen dendritic cells


ABSTRACT: Mouse splenic dendritic cells are divided into different subsets based on their phynotype. CD8α+ and CD8α- dendritic cells play different roles against pathogens. Cross-presentation is essential for immune defense against viruses, tumors and intracellular bacteria and CD8α+ DCs are more potent in cross-presentation compared to CD8α- DCs. We used microarrays to detail the global gene expression to analyze the underlying mechanism in CD8α+ dendritic cells cross-presentation Splenic CD8α+ and CD8α- dendritic cells were sorted by flow-cytometer for RNA extraction and hybridization on Affymetrix microarrays. Thirty C57BL/6J mice were sacrified for the splenic cells, splenic DCs were enriched by negtive selection, then CD8α+ and CD8α- dendritic cells were seprated based on CD11c and CD8α markers.

ORGANISM(S): Mus musculus

SUBMITTER: Zhenhong Guo 

PROVIDER: E-GEOD-64124 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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