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Downstream genes regulated by TSHZ2 in MCF-7 cells

ABSTRACT: To clarify downstream genes regulated by TSHZ2, MCF-7 cells were lentivirally transduced by TSHZ2 and its control LacZ and served their RNA samples for gene expression analysis using AGILENT human 8x60K cDNA microarray. Array analysis of MCF-7 cells expressing either TSHZ2 or LacZ. 1 color method was employed.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenji Kasai

PROVIDER: E-GEOD-64351 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Down-regulation of the zinc-finger homeobox protein TSHZ2 releases GLI1 from the nuclear repressor complex to restore its transcriptional activity during mammary tumorigenesis.

Riku Miho M Inaguma Shingo S Ito Hideaki H Tsunoda Takumi T Ikeda Hiroshi H Kasai Kenji K

Oncotarget 20160201 5

Although breast cancer is one of the most common malignancies, the molecular mechanisms underlying its development and progression are not fully understood. To identify key molecules involved, we screened publicly available microarray datasets for genes differentially expressed between breast cancers and normal mammary glands. We found that three of the genes predicted in this analysis were differentially expressed among human mammary tissues and cell lines. Of these genes, we focused on the rol ...[more]

PMID: 26744317

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GLI1-regulated genes in human mammary epithelial cells and human breast cancer cells

Project description:To clarify transcriptional target genes of GLI1 in human mammary epithelial cells and breast cancer cells, primary culture cells of human mammary epithelium HMEC and breast cancer cell line MCF-7 were lentivirally transduced by either GLI1 or its control LacZ. Their RNA samples were served for expression analysis using AGILENT human 8x60K cDNA microarray. Array analysis of HMEC and MCF-7 expressing either GLI1 or LacZ. 1 color method was employed.

2015-04-28 | E-GEOD-64350 | biostudies-arrayexpress

Gene expression profiling in MCF-7 cells exposed to blackcurrant extract.

Project description:Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) Î±. Microarray and ingenuity pathway analysis showed that BCE activated upstream genes such as ERÎ± and transforming growth factor beta 1, and upregulated the expression of many genes downstream of ERÎ±. MCF-7 cells were seeded in culture dish and maintain to confluent. Then medium replace with phenol-red-serum-free DMEM medium with or without BCE (50Î¼g/ml). After the cells were incubated for 24 h at 37 Â°C 5% CO2.

2014-06-10 | E-GEOD-58304 | biostudies-arrayexpress

Effect of cajanin stilbene acid and its synthetic derivatives on MCF-7 breast cancer cells

Project description:Cajanin stilbene acid (CSA) was previously shown to induce cytotoxicity against cancer cells. We have employed microarray gene expression profiling to identify deregulated genes in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). TGM2 gene encoding transglutaminase 2 was commonly deregulated after treatment with all four CSA compounds. All compounds revealed strong effects on cell cycle progression and DNA damage response. Promoter motif analysis of the deregulated genes further supported the microarray results emphasizing the relevance of transcription factors regulating cell cycle and proliferation, MYC being among the most pronounced ones. Interestingly, cellular movement was among the top five affected cellular functions after treatment with the four derivatives. Gene expression profiling study with Whole Human Genome RNA chips (8M-CM-^W60K Agilent) was performed in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). Twelve chips were used; control (x2), CSA (x2), control (x2), CSA6 (x2), CSA9 (x2), CSA19 (x2). MCF-7 cells were treated with the compounds (IC50 concentrations) for 72 hours or left untreated (DMSO control). Two independent experiments were performed for each treatment.

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2015-04-27 | E-GEOD-68311 | biostudies-arrayexpress

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