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Induction of circular RNA in fetal heart development recapitulated in in vitro differentiation

ABSTRACT: We discovered induction of circular RNA in human fetal tissues, including the heart. In this study, we were able to recapitulate this induction by in vitro directed differentiation of hESCs to cardiomyocytes, paving the way for future studies into circular RNA regulation. We harvested hESCs at sequential stages of differentiation: undifferentiated (day 0), mesoderm (day 2), cardiac progenitor (day 5) and definitive cardiomyocyte (day 14). We performed RNA sequencing in biological triplicate, with 3-8 technical replicates each.

ORGANISM(S): Homo sapiens

SUBMITTER: Linda Szabo

PROVIDER: E-GEOD-64417 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development.

Szabo Linda L Morey Robert R Palpant Nathan J NJ Wang Peter L PL Afari Nastaran N Jiang Chuan C Parast Mana M MM Murry Charles E CE Laurent Louise C LC Salzman Julia J

Genome biology 20150616

<h4>Background</h4>The pervasive expression of circular RNA is a recently discovered feature of gene expression in highly diverged eukaryotes, but the functions of most circular RNAs are still unknown. Computational methods to discover and quantify circular RNA are essential. Moreover, discovering biological contexts where circular RNAs are regulated will shed light on potential functional roles they may play.<h4>Results</h4>We present a new algorithm that increases the sensitivity and specifici ...[more]

PMID: 26076956

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