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Enhancer divergence and cis-regulatory evolution in the human and chimpanzee neural crest

ABSTRACT: Here we derive human and chimpanzee cranial neural crest cells (CNCCs) and profile histone modifications, transcription factors, chromatin accessibility and gene expression to systematically and quantitatively annotate evolutionary divergence of craniofacial cis-regulatory landscapes. Histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), chromatin modifiers (p300), transcription factors (NR2F1, TFAP2A), chromatin accessibility (ATACseq) and gene expression (RNAseq) were assayed in CNCCs derived from iPSCs/ESCs from 2 chimpanzee and 3 human individuals.

ORGANISM(S): Homo sapiens

SUBMITTER: Joanna Wysocka

PROVIDER: E-GEOD-70751 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Enhancer divergence and cis-regulatory evolution in the human and chimp neural crest.

Prescott Sara L SL Srinivasan Rajini R Marchetto Maria Carolina MC Grishina Irina I Narvaiza Iñigo I Selleri Licia L Gage Fred H FH Swigut Tomek T Wysocka Joanna J

Cell 20150910 1

cis-regulatory changes play a central role in morphological divergence, yet the regulatory principles underlying emergence of human traits remain poorly understood. Here, we use epigenomic profiling from human and chimpanzee cranial neural crest cells to systematically and quantitatively annotate divergence of craniofacial cis-regulatory landscapes. Epigenomic divergence is often attributable to genetic variation within TF motifs at orthologous enhancers, with a novel motif being most predictive ...[more]

PMID: 26365491

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