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Unknown,Transcriptomics,Genomics,Proteomics

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Differential gene regulation by the disease-associated short isoform of GATA1 (microarray)

ABSTRACT: The transcriptional activiy of GATA1s was compared to GATA1 through gene expression analysis in a cell line model with both erythroid and megakaryocyte differentiation. G1ME cells were derived from Gata1- mouse ES cells and have both megakaryocyte and erythrocyte differentiation potential upon reconstitution of GATA-1 expression (Stachura 2006). HA-tagged full length or short GATA-1 were expressed in G1ME cells grown in TPO via retroviral transductions. The cells were sorted for GFP positivity 68 hours post-transduction and then were allowed to recover in normal growth medium for 4h. Total RNA was then isolated using RNeasy kit from Qiagen 72 hours post-transduction.

ORGANISM(S): Mus musculus

SUBMITTER: Timothy Chlon

PROVIDER: E-GEOD-64494 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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