Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: James Mohan
PROVIDER: E-GEOD-64508 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Scientific reports 20150902
The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic β-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, β-cells could be quickly isolated by flow cytometry and were >99% insulin positive. These studies were confirmed by immunostaining, microscopy and gene expression profiling ...[more]