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Transcription profiling of mouse postnatal day 1 and adult bladders

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Experiment Overall Design: Bladders, from postnatal day 1 and adult SMGA/EGFP animals were isolated by dissection and total RNA extracted for gene expression analysis using the Affymetrix MOE430 microarray chip.

ORGANISM(S): Mus musculus

SUBMITTER: Mehran Sharghi

PROVIDER: E-GEOD-6466 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of mouse embryonic day 14 bladder, urogenital sinus, and urethra.

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination ofmicrodissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Experiment Overall Design: Bladder, urogenital sinus, and urethra regions from embryonic day 14 SMGA/EGFP animals were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-14 | E-GEOD-6223 | biostudies-arrayexpress

Transcription profiling of mouse neonatal bladder samples. (GUDMAP Series_id: 19)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of FACS sorted newborn bladder cells and compares two distinct cell populations of smooth muscle cells. Experiment Overall Design: Sorted bladder cells and bladder tissue from newborn SMGA/EGFP and SMAA/EYFP animals were microdissected or laser captured and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Transcription profiling of mouse postnatal day 1 ureter (GUDMAP Series_id: 14)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital tract. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of postnatal day 1 ureters. Experiment Overall Design: Ureters were isolated from postnatal day 1 SMGA/EGFP transgenic mice and RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of compartmental bladder tissues collected through laser capture microscopy. Experiment Overall Design: Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isloated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 degrees C until RNA was extracted for gene expression analysis. Laser captured and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Transcription profiling of mouse SMGA/EGFP adult ureter. (GUDMAP Series_id: 15)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult ureter. Experiment Overall Design: Ureter regions from adult SMGA/EGFP animals were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

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Gene expression profiles of E14 bladder, urogenital sinus and urethra isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 6)

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Gene expression profiles of neonatal bladder samples. (GUDMAP Series ID: 19)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of FACS sorted newborn bladder cells and compares two distinct cell populations of smooth muscle cells since both of these populations contain EGFP from a SMGA (Actg2) promoter shown to be expressed only in smooth muscle cells. Gene expression comparison from developing regions of the mouse postnatal day 2 urogenital system.

2008-04-17 | GSE11161 | GEO

Transcription profiling of mouse P7 bladder epithelium compartments: uroplakin expressing (+ve) vs. non-expressing (-ve) epithelial cells (GUDMAP Series_id: 34)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the epithelial compartments of the P7 mouse bladder. Experiment Overall Design: At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in TyrodeÃs solution for 30 minutes at 37 degrees C. The layers were separated by gentle microdissection and the epithelial layer was further digested in a mixture of Blendzymes1/4 at 37 degrees C until a single cell suspension was obtained The samples were placed in RLT and stored at -80 degrees C.RNA was prepared and the Epicenter 2-round amplification scheme described on the web was performed. The Affymetrix GeneChip Mouse Genome 430 2.0 Array was used to interrogate the amplified RNA.

2010-02-17 | E-GEOD-19979 | biostudies-arrayexpress

Transcription profiling of mouse embryonic day 14 gential tubercle of the male. (GUDMAP Series_id: 16)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 gential tubercle of the male. Experiment Overall Design: The genital tubercle region from an E14 (TS22) SMAA/EYFP males were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-15 | E-GEOD-7903 | biostudies-arrayexpress

Gene expression profiles of E13 bladder neck/urethral compartments (GUDMAP Series ID: 25)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of micro dissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the mesenchymal and epithelial compartments of the E13 mouse bladder neck/urethral compartment. FVB/N mice were time mated. At embryonic day 13 mice were euthanized by decapitation and the bladders were microdissected and cut just below the ureters. Since the EDTA dissociation was ineffective, the bladder neck and urethra were collected and treated with 1 mg/ml trypsin in Tyrode's solution for 20 minutes at 37°C. The layers were separated using a fine needle and rimming. The samples were placed in RLT and stored at -80°C. RNA was prepared and the Epicentre 2-round amplification scheme described under the Lessard Group Protocols on the GUDMAP pages were performed. The amplified RNA was examined with the Affymetrix GeneChip Mouse Genome 430 2.0 Array.

2009-04-24 | E-GEOD-15794 | biostudies-arrayexpress

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