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Transcription profiling of mouse SMGA/EGFP adult ureter. (GUDMAP Series_id: 15)

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult ureter. Experiment Overall Design: Ureter regions from adult SMGA/EGFP animals were microdissected and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

ORGANISM(S): Mus musculus

SUBMITTER: Mehran Sharghi

PROVIDER: E-GEOD-7887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiles of adult ureter isolated from SMGA (Actg2) transgenic mice. (GUDMAP Series ID: 15)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing urogenital system. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the adult ureter. Keywords: Gene expression analysis of adult mouse ureter

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2008-06-15 | E-GEOD-6963 | biostudies-arrayexpress

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Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the e15 mouse ureter. Comparisons with the same compartments in the neonate will be made to determine changes in gene expression from relatively undifferentiated tissue (e15) to a highly differentiated structure (neonate).

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2008-06-14 | E-GEOD-6223 | biostudies-arrayexpress

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Transcription profiling of mouse postnatal day 1 and adult bladders

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of the embryonic day 14 bladder, urogenital sinus, and urethra. Experiment Overall Design: Bladders, from postnatal day 1 and adult SMGA/EGFP animals were isolated by dissection and total RNA extracted for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-14 | E-GEOD-6466 | biostudies-arrayexpress

Transcription profiling of mouse neonatal bladder samples. (GUDMAP Series_id: 19)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of FACS sorted newborn bladder cells and compares two distinct cell populations of smooth muscle cells. Experiment Overall Design: Sorted bladder cells and bladder tissue from newborn SMGA/EGFP and SMAA/EYFP animals were microdissected or laser captured and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-11 | E-GEOD-11161 | biostudies-arrayexpress

Transcription profiling of mouse detrusor, stroma and urothelium from neonatal bladder. (GUDMAP Series_id: 20)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here represents the gene expression profiles of compartmental bladder tissues collected through laser capture microscopy. Experiment Overall Design: Bladders were isolated from newborn SMGA/EGFP transgenic mice, embedded in OCT, frozen and sectioned (8 microns). Detrusor, stroma and urothelium were isloated using laser capture microscopy. The caps were frozen on dry ice and stored at -80 degrees C until RNA was extracted for gene expression analysis. Laser captured and total RNA isolated for gene expression analysis using the Affymetrix MOE430 microarray chip.

2008-06-11 | E-GEOD-11221 | biostudies-arrayexpress

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