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RNA sequencing of ATCC 17978hm Acineotbacter baumannii strain and CpdA mutant

ABSTRACT: Difference in RNA expression levels between Acinetobacter baumannii cells expressing high and low levels of cyclic AMP Total RNA obtained from Acineotbacter baumannii bacterial cells in Log phase gown in MH broth culture, isolated RNA in triplicate from three expreiment. cpdA::Tn mutant and 17978hm strain compared. Assessing increased levels of cAMP within the cell

ORGANISM(S): Acinetobacter baumannii

SUBMITTER: Uwe Stroeher

PROVIDER: E-GEOD-64935 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes essential for pellicle formation in Acinetobacter baumannii.

Giles Sarah K SK Stroeher Uwe H UH Eijkelkamp Bart A BA Brown Melissa H MH

BMC microbiology 20150606

<h4>Background</h4>Acinetobacter baumannii is an opportunistic pathogen, which has the ability to persist in the clinical environment, causing acute and chronic infections. A possible mechanism contributing to survival of A. baumannii is its ability to form a biofilm-like structure at the air/liquid interface, known as a pellicle. This study aimed to identify and characterise the molecular mechanisms required for pellicle formation in A. baumannii and to assess a broad range of clinical A. bauma ...[more]

PMID: 26047954

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Project description:Characterization of Nalpha- and Nexpsilon-acetylation in Acinetobacter baumannii ATCC 17978 using different analytical approaches (isoelectrofocusing, strong cation exchange, immunoprecipitation, CNBr enrichment, and high resolutive and accurate mass spectrometry).

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Combined analysis of genome-wide DNA methylome and transcriptome reveals the first epigenetic-based antibiotic-resistance mechanism in Acinetobacter baumannii

Project description:Using Nanopore sequencing, our study has revealed a close correlation between genomic methylation levels and antibiotic resistance rates in Acinetobacter Baumannii. Specifically, the combined genome-wide DNA methylome and transcriptome analysis revealed the first epigenetic-based antibiotic-resistance mechanism in A. baumannii. Our findings suggest that the precise location of methylation sites along the chromosome could provide new diagnostic markers and drug targets to improve the management of multidrug-resistant A. baumannii infections.

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