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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

ABSTRACT: The analysis compares primary fibroblasts initially used for reprogramming, established marmoset ES cells and a marmoset iPS cell line which was generated witha non-viral approach using a six-factor-in-one-vector approach Cells were grown under standard conditions for marmoset pluripotent stem cells and primary fibroblasts without additional treatment. For each cell type at least four independent cell preparations were used.

ORGANISM(S): Callithrix jacchus

SUBMITTER: Gabriela Salinas-Riester

PROVIDER: E-GEOD-64966 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach.

Debowski Katharina K Warthemann Rita R Lentes Jana J Salinas-Riester Gabriela G Dressel Ralf R Langenstroth Daniel D Gromoll Jörg J Sasaki Erika E Behr Rüdiger R

PloS one 20150318 3

Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem (iPS) cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived functional cells such as neurons may become the key for an effective treatment of currently incurable degenerative dise ...[more]

PMID: 25785453

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