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The effect of THADA knockdown on Huh7 cells

ABSTRACT: We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells. The pGIPZ shRNA plasmid specific to human THADA (clone ID V3LHS_318037 Open Biosystems, Inc.) or the pGIPZ non-silencing shRNAmir lentiviral plasmid (as a control) was cotransfected with the pPACKH1 plasmid mix in HEK 293 cells. Stable clones of Huh7 cells with THADA knockdown were routinely maintained in a medium containing of puromycin (500ng/ml)

ORGANISM(S): Homo sapiens

SUBMITTER: Takafumi Yoshida

PROVIDER: E-GEOD-65233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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