Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Global transcriptomic changes induced by manipulate MSH1 gene in tomato using next generation sequencing

ABSTRACT: apical meristem tissues from 4-week-old wild type, epiF3, MSH1 gene mutant including (mild-DR and dwarf-DR) plants were used in this experiment. For each genotype, the transcriptome was sequenced with 3 biological replicates.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: xiaodong yang

PROVIDER: E-GEOD-65242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MutS HOMOLOG1-derived epigenetic breeding potential in tomato.

Yang Xiaodong X Kundariya Hardik H Xu Ying-Zhi YZ Sandhu Ajay A Yu Jiantao J Hutton Samuel F SF Zhang Mingfang M Mackenzie Sally A SA

Plant physiology 20150303 1

Evidence is compelling in support of a naturally occurring epigenetic influence on phenotype expression in land plants, although discerning the epigenetic contribution is difficult. Agriculturally important attributes like heterosis, inbreeding depression, phenotypic plasticity, and environmental stress response are thought to have significant epigenetic components, but unequivocal demonstration of this is often infeasible. Here, we investigate gene silencing of a single nuclear gene, MutS HOMOL ...[more]

PMID: 25736208

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Project description:Tomato (Solanum lycopersicum) is a model species for studying fruit development, wounding, herbivory, and pathogen attack. Despite tomato’s world-wide economic importance and the role of chloroplasts as metabolic hubs and integrators of environmental cues, little is known about the stromal proteome of tomato. Using a high-yielding protocol for chloroplast and stromal protein isolation, MudPIT nano-LC-MS/MS analyses, a robust in-house protein database (the Atlas) for predicting the plastid localization of tomato proteins, and rigorous selection criteria for inclusion/exclusion in the stromal proteome, we identified 1,254 proteins of the tomato stromal proteome. We provide one of the most robust stromal proteomes available to date with empirical evidence for 550 and 105 proteins not previously described for tomato plastids and the Arabidopsis stroma, respectively. The relative abundance of tomato stromal proteins was determined using the exponentially modified protein abundance index (emPAI). Comparison of the abundance of tomato and Arabidopsis stromal proteomes provided evidence for the species-specific nature of stromal protein homeostasis. The manual curation of the tomato stromal proteome classified proteins into ten functional categories resulting in an accessible compendium of tomato chloroplast proteins. After curation, only 88 proteins remained as unknown, uncharacterized or as enzymes with unknown functions. The curation of the tomato stromal proteins also indicated that tomato has a number of paralogous proteins, not present in Arabidopsis, which accumulated to different levels in chloroplasts. As some of these proteins function in key metabolic pathways or in perceiving or transmitting signals critical for plant adaptation to biotic and abiotic stress, these data suggest that tomato may modulate the bidirectional communication between chloroplasts and nuclei in a novel manner. The stromal proteome provides a fertile ground for future mechanistic studies in the field of tomato chloroplast-nuclear signaling and are foundational for our goal of elucidating the dynamics of the stromal proteome controlled by the solanaceous-specific, stromal, and wound-inducible leucine aminopeptidase A of tomato.

Dataset Information

Global transcriptomic changes induced by manipulate MSH1 gene in tomato using next generation sequencing

Publications

MutS HOMOLOG1-derived epigenetic breeding potential in tomato.

Similar Datasets

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