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Macrophage gene expression associated with remodeling of the prepartum rat cervix: Microarray and pathway analyses

ABSTRACT: As the critical gatekeeper for birth, prepartum remodeling of the cervix is associated with increased resident macrophages (MM-OM-^F), proinflammatory processes, and extracellular matrix degradation. This study tested the hypothesis that expression of genes unique to MM-OM-^Fs characterizes the prepartum from unremodeled nonpregnant cervix. Perfused cervix from prepartum day 21 postbreeding (D21) or nonpregnant (NP) rats, with or without MM-OM-^Fs, had RNA extracted and whole genome microarray analysis performed. By subtractive analyses, expression of 194 and 120 genes related to MM-OM-^Fs in the cervix from D21 rats were increased and decreased, respectively. In both D21 and NP groups, 158 and 57 MM-OM-^F genes were also more or less up- or down-regulated, respectively. Gene expression from four groups: D21 pregnant or non-pregnant rat cervix, with or without macrophages. Comparing the with and without macrophage groups can show genes that are expressed only in macrophages.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Abigail Dobyns

PROVIDER: E-GEOD-65265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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