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ChIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

ABSTRACT: Whole genome profiling of histone methylation (H3K4me3, H3K27me3 and RNA Pol II) in Ciliary derived stem/progenitor cells and differentiated cells. In the present study we focus on the cellular differentiation that involves loss of pluripotency and gain of lineage and cell type-specific characteristics and we show developments from adult ciliary derived stem/progenitor cells to differentiated retinal neurons/glial cells of human cadaveric eyes in vitro. Ciliary pigment epithelial cells isolated from human cadaveric eye balls were cultured in the presence of mitogens like Epidermal Growth Factor (EGF) and Fibroblast Growth Factors (FGF) to generate neurospheres (Stem/progenitor population). The generated neurospheres were plated on laminin and poly-D-lysine-coated cover slips in the presence of the Brain derived neurotrophic factor (BDNF), Retinoic acid (RA) and 10% FBS for the differentiation (Retinal neuron/glial cells). Global Methylation patterns of histone H3K4me3, RNA Pol II and H3K27me3 were analysed in the present study. This work would provide an outline of epigenetic modifications in ciliary derived stem/progenitor cells and the progeny that underwent differentiation into retinal neurons/glial cells and present that specific histone methylations are involved in gene expression reprogramming during the process of differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Genotypic technology

PROVIDER: E-GEOD-65268 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

Project description:Whole genome profiling of histone methylation (H3K4me3, H3K27me3 and RNA Pol II) in Ciliary derived stem/progenitor cells and differentiated cells. In the present study we focus on the cellular differentiation that involves loss of pluripotency and gain of lineage and cell type-specific characteristics and we show developments from adult ciliary derived stem/progenitor cells to differentiated retinal neurons/glial cells of human cadaveric eyes in vitro.

2016-01-02 | GSE65268 | GEO

MeDIP-on-Chip for the Ciliary derived stem/progenitor cells and their differentiated progeny.

Project description:Whole genome profiling of DNA methylation in Ciliary derived stem/progenitor cells and differentiated cells 6 samples total, 3 with CPE derived neurospheres and 3 with CPE derived differentiated progeny.

2016-07-04 | E-GEOD-64546 | biostudies-arrayexpress

Whole gene expression profiling: A study for analysing the gene expression of the retinal stem cells and the differentiated cells

Project description:Comparitive analysis of the retinal stem/progentior cells derived from the ciliary and iris pigment epithelial cells and the subsequent differentiated cells derived from the stem/progenitor cells. Gene expression profiling has shown great promise in analysing the reprogramming pattern of the cells under various culture conditions, in this context we analysed the various differential gene expression pattern of the neurospheres derived from the ciliary and iris pigment epithelial cells, and their differentiated cells. This provide an insite for the stem cell transplantation studies. We analysed 3 biological cultures derived from each catogory like the primary cells of both ciliary and iris pigment epithelial cells, the stem/progenitor cells (Neurospheres), neurosphere derived differentiated cells. No technical replicates were performed.

2010-07-31 | E-GEOD-23340 | biostudies-arrayexpress

Optic Vesicle Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment

Project description:Microarray anlaysis was performed to investigate gene expression patterns of other transcription factors involved in early retinal and/or forebrain development using human embryonic stem cell-derived retinal and forebrain progenitor cells. After 20 days of differentiation, vesicular neurospheres selectively expressed multiple retinal transcription factor genes appropriate for the OV stage of retinogenesis, whereas nonvesicular neurospheres expressed transcription factors indicative of the embryonic forebrain. Many transcription factor genes associated with retinal development were present at higher levels in vesicular vs. nonvesicular neurospheres. Nonvesicular neurospheres, on the other hand, expressed higher levels of transcription factors implicated in early forebrain development. Taken together, results indicated that the vesicular and nonvesicular neurospheres harbored retinal progenitor cells and early forebrain populations, respectively. Compare the global gene expression of retinal progenitor cells and forebrain progenitor cells derived from human embryonic stem cells

2011-12-01 | E-GEOD-29237 | biostudies-arrayexpress

Optic Vesicle Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment

2011-12-01 | GSE29237 | GEO

Whole gene expression profiling: A study for analysing the gene expression of the retinal stem cells and the differentiated cells

2010-07-31 | GSE23340 | GEO

Six3 and Six6 jointly regulate the identities and developmental trajectories of multipotent retinal progenitor cells in the embryonic mouse retina

Project description:Regulation of retinal differentiation in mammals is not adequately understood. Using single-cell RNA sequencing of control and Six3 and Six6 compound-mutant mouse embryonic eye-cups, we identified cell clusters and developmental trajectories jointly regulated by transcription factor Six3 and its close paralog Six6. In control retinas, naïve retinal progenitor cells had two major trajectories leading to ciliary margin cells and retinal neurons, respectively. The ciliary margin trajectory was directly from naïve retinal progenitor cells at G1 phase whereas the neuronal trajectory was through a neurogenic state marked by Atoh7 expression. Upon Six3 and Six6 dual deficiencies, both naïve and neurogenic retinal progenitors were defective, ciliary margin differentiation was enhanced, and multi-lineage neuronal differentiation was disrupted. An ectopic neuronal trajectory lacking the Atoh7+ state led to ectopic neurons. Additionally, opposing gradients of Wnt and Fgf signaling were perturbed. Our findings provide deeper insight into molecular mechanisms underlying early retinal differentiation in mammals.

2024-08-03 | GSE241946 | GEO

Transcription profiling of human and mouse first passage vs. primary retinal sphere cells

Project description:The pigmented portion of ciliary epithelium in the adult mammalian eye harbors mitotically quiescent retinal sphere cells, which are capable of self-renewal and differentiating into retinal neurons when assayed in vitro; however, very little is known about the molecular mechanism controlling the proliferation and differentiation of these adult retinal stem cells or their molecular resemblance to mutipotent stem/progenitor cells during early eye development. This experiment studies the gene expression of first passage and primary human and mouse retinal sphere cells. Experiment Overall Design: this experiment include 4 samples and 12 replicates

2008-06-12 | E-GEOD-3791 | biostudies-arrayexpress

Gene Expression Comparison of First Passage vs. Primary Human and Mouse Retinal Sphere Cells

2005-12-09 | GSE3791 | GEO

miRNA expression data from CD73-positive Rods, MÃ¼ler glia and E 17.5 Retinal Progenitors

Project description:During retinal development, progenitor cells give rise to six different types of neurons, and one glial cell. This process requires the expression of genes that confer specific functions and identity to each cell. Previous works have reported the miRNAs expression profile in retina, but is still necessary to further define individual retinal cell populations profiles. We isolated postmitotic mice CD73-positive Rods, MÃ¼ller glial cells and Retinal Progenitors Cells (E17.5); we then analyzed their miRNA profile expression by microarrays. Using wild type mice we FACS-isolated postmitotic CD73-positive Rods (posnatal day 5). We isolated primary MÃ¼ller glia cultures from postnatal day 8 mice and retinal progenitors cells from E17.5 mouse embryos; we then sent the samples to the gene expression unit at Instituto de Medicina Genomica (INMEGEN), to analyze their miRNA profile expression by microarrays.

2016-02-09 | E-GEOD-62109 | biostudies-arrayexpress

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