Project description:Deep sequencing of samples from different development stages, different adult organs and different stress treatments of Pacific oyster Crassostrea gigas Samples of 38 developmental stages from egg to juvenile were analyzed using single-end 49 bp RNA-seq. Two libraries mixed by RNAs from different developmental stages were analyzed using paired-end 90 bp RNA-seq. A total of 11 samples mainly from 8 organs (mantle, gill,adductor muscle, digestive gland, hemocyte, labial palp, female gonad and male gonad) were analyzed using paired-end 90 bp RNA-seq. At the same time, single-end 49 bp RNA-seq was conducted on 61 samples collected from adult oysters subjected to 9 types of environmental stressors (exposure to air, salinity, temperature, and exposure to metals).
Project description:Deep sequencing of mRNA from two Ants: Camponotus floridanus and Harpegnathos saltator Analysis of poly(A)+ RNA of different specimens: egg,larva,male,minor and major for Camponotus floridanus while egg,larva,male,worker and gammergate for Harpegnathos saltator
Project description:Deep sequencing of mRNA from queen and virgin queen of two Ants: Camponotus floridanus and Harpegnathos saltator Total RNA were isolated from virgin queens and queens by pulverizing the entire animal after removal of gut and poison glands in liquid N2 and then dissolving in TRIzol. PolyA+ RNA were prepared for deep sequencing.
Project description:Deep sequencing of mRNA from the rock pigeon Analysis of ploy(A)+ RNA of different specimens: heart and liver from the rock pigeon (Danish Tumbler, Oriental Frill and Racing)
Project description:Deep sequencing of mRNA from Fusarium oxysporum f. sp. Cubense 1 and 4 after infecting Musa acuminata 0h and 48h. Analysis of ploy(A)+ RNA of different hours after infecting of Musa acuminata
Project description:Deep sequencing of mRNA from two macaques, crab-eating macaque and Indian rhesus macaque Analysis of ploy(A)+ RNA of different specimens:brain,ileum,kidney,liver,testes and white adipose for crab-eating macaque while brain,heart,,kidney,liver,quadriceps and testes for Indian rhesus macaque
Project description:Deep sequencing of mRNA from naked mole rat Analysis of ploy(A)+ RNA of different specimens: brain, kidney, liver from new born , 4 years old , 20 years old and 4 years old hypoxia-exposed naked mole rat
Project description:Deep sequencing of mRNA from Pacific oyster Crassostrea gigas Competent larvae of Crassostrea gigas were treated with epinephrine solution, and then sampled at different time intervals. For shell damage experiment, shell were broken and then tissues were sampled at different time intervals.
Project description:Deep sequencing of mRNA from 6 organs of yak (Bos grunniens) Analysis of ploy(A)+ RNA of brain,heart,liver,lung,spleen, and stomach of yak (Bos grunniens)