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Transcription profiling of human embryonic stem cell line containining HSR H14, and parent H14 l

ABSTRACT: Homogenous staining regions (hsr) are cytogenetic representations of a gene amplification. They are found exclusively in tumour cells. We found a hsr in the human embryonic stem cell (hESC) line H14, this is the first report of a hsr in hESCs. FISH and CGH studies showed that the hsr was derived from chromosome 17p11.2. The gene expression analysis studies were performed to identify the genes upregulated due to the hsr by comparison the the hsr-contianing H14 cells with a karyotypically normal parent H14 cell line. Experiment Overall Design: H14 HSR positive embryonic stem cells and H14 HSR negative cells were hybridised to Affymetrix U133 Plus 2.0 expression arrays. Replicates were also run using H14 positive and H14 negative cells from different passages.

ORGANISM(S): Homo sapiens

SUBMITTER: Duncan Baker

PROVIDER: E-GEOD-6561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Adaptation to culture of human embryonic stem cells and oncogenesis in vivo.

Baker Duncan E C DE Harrison Neil J NJ Maltby Edna E Smith Kath K Moore Harry D HD Shaw Pamela J PJ Heath Paul R PR Holden Hazel H Andrews Peter W PW

Nature biotechnology 20070201 2

The application of human embryonic stem cells (HESCs) to provide differentiated cells for regenerative medicine will require the continuous maintenance of the undifferentiated stem cells for long periods in culture. However, chromosomal stability during extended passaging cannot be guaranteed, as recent cytogenetic studies of HESCs have shown karyotypic aberrations. The observed karyotypic aberrations probably reflect the progressive adaptation of self-renewing cells to their culture conditions. ...[more]

PMID: 17287758

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