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Max as a biological blockade that restricts meiotic process in ESCs

ABSTRACT: We explored Max ablation-mediated up-regulation of germ-related genes, especially meiosis-related genes in mouse embryonic stem cells which were cultured either under conventional mouse ES medium or 2i condition using inhibitors against MEK and GSK3b. Effect of culture conditions (conventional mouse ES medium or 2i condition) on the expression profile of germ-related genes in Max expression ablated ES cells were examined using inducible Max-null ES cells in which Max gene had been homozygously disrupted, but carries Max cDNA in ROSA26 locus with tetracycline-off system.

ORGANISM(S): Mus musculus

SUBMITTER: Akihiko Okuda

PROVIDER: E-GEOD-65700 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells.

Suzuki Ayumu A Hirasaki Masataka M Hishida Tomoaki T Wu Jun J Okamura Daiji D Ueda Atsushi A Nishimoto Masazumi M Nakachi Yutaka Y Mizuno Yosuke Y Okazaki Yasushi Y Matsui Yasuhisa Y Izpisua Belmonte Juan Carlos JC Okuda Akihiko A

Nature communications 20160330

Meiosis is a unique process that allows the generation of reproductive cells. It remains largely unknown how meiosis is initiated in germ cells and why non-germline cells do not undergo meiosis. We previously demonstrated that knockdown of Max expression, a gene encoding a partner of MYC family proteins, strongly activates expression of germ cell-related genes in ESCs. Here we find that complete ablation of Max expression in ESCs results in profound cytological changes reminiscent of cells under ...[more]

PMID: 27025988

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