PAPERCLIP identifies novel microRNA targets and provides insights into mRNA alternative polyadenylation
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ABSTRACT: Accurate and precise annotation of the 3Ⲡuntranslated regions (3ⲠUTRs) is critical in understanding how mRNA stability, localization and translational efficiency are regulated by microRNAs (miRNAs) and RNA-binding proteins (RBPs). Here we describe a novel method, PAPERCLIP (Poly(A) binding Protein-mediated mRNA 3´End Retrieval by CrossLinking ImmunoPrecipitation), which shows high specificity for the mRNA 3Ⲡends and compares favorably to existing 3Ⲡend mapping methods including direct RNA sequencing in performance. PAPERCLIP protocol and RNA-Seq on human cell lines and mouse whole brain cortex.
ORGANISM(S): Mus musculus
SUBMITTER: Hun-Way Hwang
PROVIDER: E-GEOD-66092 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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