Unknown,Transcriptomics,Genomics,Proteomics

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PAPERCLIP identifies novel microRNA targets and provides insights into mRNA alternative polyadenylation


ABSTRACT: Accurate and precise annotation of the 3′ untranslated regions (3′ UTRs) is critical in understanding how mRNA stability, localization and translational efficiency are regulated by microRNAs (miRNAs) and RNA-binding proteins (RBPs). Here we describe a novel method, PAPERCLIP (Poly(A) binding Protein-mediated mRNA 3´End Retrieval by CrossLinking ImmunoPrecipitation), which shows high specificity for the mRNA 3′ ends and compares favorably to existing 3′ end mapping methods including direct RNA sequencing in performance. PAPERCLIP protocol and RNA-Seq on human cell lines and mouse whole brain cortex.

ORGANISM(S): Mus musculus

SUBMITTER: Hun-Way Hwang 

PROVIDER: E-GEOD-66092 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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