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Sex comb on midleg (Scm) is a functional link between PcG repressive complexes in Drosophila

ABSTRACT: We profiled Sex comb on midleg (Scm), Pc and E(z) in fly embryos and S2 based on BioTAP-XL ChIP-seq. ChIP-seq revealed that Scm is co-localized with PRC1, PRC2, and H3K27me3 in both S2 cells and embryos. Genomic binding/occupancy profiling of Scm, Pc and E(z) by high throughput sequencing

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Peter Park

PROVIDER: E-GEOD-66183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.

Kang Hyuckjoon H McElroy Kyle A KA Jung Youngsook Lucy YL Alekseyenko Artyom A AA Zee Barry M BM Park Peter J PJ Kuroda Mitzi I MI

Genes & development 20150601 11

The Polycomb group (PcG) proteins are key regulators of development in Drosophila and are strongly implicated in human health and disease. How PcG complexes form repressive chromatin domains remains unclear. Using cross-linked affinity purifications of BioTAP-Polycomb (Pc) or BioTAP-Enhancer of zeste [E(z)], we captured all PcG-repressive complex 1 (PRC1) or PRC2 core components and Sex comb on midleg (Scm) as the only protein strongly enriched with both complexes. Although previously not linked ...[more]

PMID: 26063573

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