Chromatin signatures at Notch regulated enhancers reveal large-scale changes in H3K56ac upon activation
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
SUBMITTER: Sarah Bray
PROVIDER: E-GEOD-66227 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Chromatin signatures at Notch-regulated enhancers reveal large-scale changes in H3K56ac upon activation.
Skalska Lenka L Stojnic Robert R Li Jinghua J Fischer Bettina B Cerda-Moya Gustavo G Sakai Hiroshi H Tajbakhsh Shahragim S Russell Steven S Adryan Boris B Bray Sarah J SJ
The EMBO journal 20150611 14
The conserved Notch pathway functions in diverse developmental and disease-related processes, requiring mechanisms to ensure appropriate target selection and gene activation in each context. To investigate the influence of chromatin organisation and dynamics on the response to Notch signalling, we partitioned Drosophila chromatin using histone modifications and established the preferred chromatin conditions for binding of Su(H), the Notch pathway transcription factor. By manipulating activity of ...[more]
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Project description:The conserved Notch pathway functions in diverse developmental and disease-related processes, requiring mechanisms to ensure appropriate target-selection and gene activation in each context. To investigate, we partitioned Drosophila chromatin into different states, based on histone modifications, establishing the preferred chromatin conditions for binding of CSL, the Notch pathway transcription factor. While most histone modifications were unchanged by CSL binding or Notch activation, rapid changes in H3K56 acetylation occurred at Notch regulated-enhancers. This modification extended over large regions, required the histone acetyl-transferase CBP and was independent of transcription. Such rapid changes in H3K56 acetylation are a conserved indicator of enhancer activation, also occurring at mammalian Notch-regulated Hey1 and at Drosophila ecdysone-regulated genes. This core histone modification may therefore underpin the changes in chromatin accessibility needed to promote transcription following signaling activation. Su(H) profile of BG3 cells in control condition and EGTA treated condition. In total 8 samples, 4 replicates of Su(H) ChIP in hbss condition and 4 replicates of Su(H) ChIP in EGTA treated BG3 cells.
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