Unknown,Transcriptomics,Genomics,Proteomics

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Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (mouse)


ABSTRACT: Murine CD45.1+ CD117+ BM cells were co-transduced pairwise with retroviral vectors expressing Hoxa9, Meis1, Foxc1 or a control empty vector. Ninety-six hours later 10^6 drug resistant cells were transplanted into CD45.2+ irradiated congenic recipients. To investigate the consequences of FOXC1 expression on the transcriptome in murine AML, we performed exon array analysis using flow sorted CD117+Gr1+ leukemia cells recovered from sick mice (Hoxa9/control; Moxa9/Meis1; or Hoxa9/FOXC1 leukemias; 3 mice per cohort). Nine exon arrays total; 3 arrays for each cohort (Hoxa9/control; Moxa9/Meis1; or Hoxa9/FOXC1), each from a separate mouse.

ORGANISM(S): Mus musculus

SUBMITTER: Tim Somervaille 

PROVIDER: E-GEOD-66253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Frequent Derepression of the Mesenchymal Transcription Factor Gene FOXC1 in Acute Myeloid Leukemia.

Somerville Tim D D TD   Wiseman Daniel H DH   Spencer Gary J GJ   Huang Xu X   Lynch James T JT   Leong Hui Sun HS   Williams Emma L EL   Cheesman Edmund E   Somervaille Tim C P TC  

Cancer cell 20150901 3


Through in silico and other analyses, we identified FOXC1 as expressed in at least 20% of human AML cases, but not in normal hematopoietic populations. FOXC1 expression in AML was almost exclusively associated with expression of the HOXA/B locus. Functional experiments demonstrated that FOXC1 contributes to a block in monocyte/macrophage differentiation and enhances clonogenic potential. In in vivo analyses, FOXC1 collaborates with HOXA9 to accelerate significantly the onset of symptomatic leuke  ...[more]

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