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MiR-431 promotes differentiation and regeneration of old skeletal muscle by targeting Smad4

ABSTRACT: we identified novel age-related microRNAs through comparative analysis of miRNA expression profiles in young and old myoblasts using next generation sequencing (NGS) small RNA profiles of young mouse and old mouse were generated by deep sequencing, in each four samples, using Illumina GAIIx

ORGANISM(S): Mus musculus

SUBMITTER: sujin Baek

PROVIDER: E-GEOD-66267 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-431 promotes differentiation and regeneration of old skeletal muscle by targeting Smad4.

Lee Kwang-Pyo KP Shin Yeo Jin YJ Panda Amaresh C AC Abdelmohsen Kotb K Kim Ji Young JY Lee Seung-Min SM Bahn Young Jae YJ Choi Jeong Yi JY Kwon Eun-Soo ES Baek Su-Jin SJ Kim Seon-Young SY Gorospe Myriam M Kwon Ki-Sun KS

Genes & development 20150727 15

The myogenic capacity of myoblasts decreases in skeletal muscle with age. In addition to environmental factors, intrinsic factors are important for maintaining the regenerative potential of muscle progenitor cells, but their identities are largely unknown. Here, comparative analysis of microRNA (miRNA) expression profiles in young and old myoblasts uncovered miR-431 as a novel miRNA showing markedly reduced abundance in aged myoblasts. Importantly, elevating miR-431 improved the myogenic capacit ...[more]

PMID: 26215566

Similar Datasets

miR-431 promotes differentiation and regeneration of old skeletal muscle by targeting Smad4

Project description:we identified novel age-related microRNAs through comparative analysis of miRNA expression profiles in young and old myoblasts using next generation sequencing (NGS)

2015-09-04 | GSE66267 | GEO

miR-431 promotes differentiation and regeneration of old skeletal muscle by targeting Smad4.

Project description:The myogenic capacity of myoblasts decreases in skeletal muscle with age. In addition to environmental factors, intrinsic factors are important for maintaining the regenerative potential of muscle progenitor cells, but their identities are largely unknown. Here, comparative analysis of microRNA (miRNA) expression profiles in young and old myoblasts uncovered miR-431 as a novel miRNA showing markedly reduced abundance in aged myoblasts. Importantly, elevating miR-431 improved the myogenic capacity of old myoblasts, while inhibiting endogenous miR-431 lowered myogenesis. Bioinformatic and biochemical analyses revealed that miR-431 directly interacted with the 3' untranslated region (UTR) of Smad4 mRNA, which encodes one of the downstream effectors of TGF-β signaling. In keeping with the low levels of miR-431 in old myoblasts, SMAD4 levels increased in this myoblast population. Interestingly, in an in vivo model of muscle regeneration following cardiotoxin injury, ectopic miR-431 injection greatly improved muscle regeneration and reduced SMAD4 levels. Consistent with the finding that the mouse miR-431 seed sequence in the Smad4 3' UTR is conserved in the human SMAD4 3' UTR, inhibition of miR-431 also repressed the myogenic capacity of human skeletal myoblasts. Taken together, our results suggest that the age-associated miR-431 plays a key role in maintaining the myogenic ability of skeletal muscle with age.

| S-EPMC4536309 | biostudies-literature

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type Young and Old muscle Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare Young and Old muscles transcriptome profiling (RNA-seq) to optimal high-throughput data analysis Results: Using an optimized data analysis workflow, we mapped about 30 million sequence reads per sample to the mouse genome (build mm10) and identified 24799 genes. Among them, 95.7% of the genes displayed the similar expression level in both young and old muscles.2% of genes (501) were significantly up-regulated and 2.3% of genes (584) were significantly down-regulated. p value <0.05.

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