Unknown,Transcriptomics,Genomics,Proteomics

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WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation


ABSTRACT: The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100. Comparison of altered gene expression in Arabidopsis WT and wrky33 mutant plants 14 hours post inoculation with Botrytis cinerea 2100.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Barbara Kracher 

PROVIDER: E-GEOD-66290 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100.

Liu Shouan S   Kracher Barbara B   Ziegler Jörg J   Birkenbihl Rainer P RP   Somssich Imre E IE  

eLife 20150615


The Arabidopsis mutant wrky33 is highly susceptible to Botrytis cinerea. We identified >1680 Botrytis-induced WRKY33 binding sites associated with 1576 Arabidopsis genes. Transcriptional profiling defined 318 functional direct target genes at 14 hr post inoculation. Comparative analyses revealed that WRKY33 possesses dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. We confirmed known WRKY33 targets involved in hormone signaling and phy  ...[more]

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