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Transcription profiling of S. cerevisiae edc3 mutant and wild type cultures

ABSTRACT: The goal of this experiment is to identify transcripts regulated by Edc3p, an activator of mRNA decapping. Experiment Overall Design: Five independent expression profiling experiments were carried out for isogenic wild-type (HFY114) and edc3Δ (CFY25) strains using Affymetrix Yeast Genome S98 Arrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Feng He

PROVIDER: E-GEOD-6647 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA.

Dong Shuyun S Li Chunfang C Zenklusen Daniel D Singer Robert H RH Jacobson Allan A He Feng F

Molecular cell 20070201 4

Autoregulatory loops often provide precise control of the level of expression of specific genes that encode key regulatory proteins. Here we have defined a pathway by which Yra1p, a yeast mRNA export factor, controls its own expression. We show that YRA1 exon 1 sequences in cis and Yra1p in trans inhibit YRA1 pre-mRNA splicing and commit the pre-mRNA to nuclear export. Mex67p and Crm1p jointly promote YRA1 pre-mRNA export, and once in the cytoplasm, the pre-mRNA is degraded by a 5' to 3' decay m ...[more]

PMID: 17317628

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Transcription profiling of S. cerevisiae to identify Upf1p-associated transcripts in S. cerevisiae

Project description:The goal of this experiment was to identify transcripts associated with the S. cerevisiae Upf1 protein. Experiment Overall Design: The RNA population from four independent pairs of Upf1p-TAP and mock affinity selections were analyzed by using Affymetrix Yeast Genome S98 Arrays.

2008-06-16 | E-GEOD-9486 | biostudies-arrayexpress

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Project description:Yeast lacking the H3 or H4 amino termini, and corresponding wild type strains, were grown in synthetic media. These conditions induce Gcn4-activated transcription. Experiment Overall Design: Three replicates of the H4del(2-26) and corresponding wild type strains, and four replicates of the H3del(1-28) and corresponding wild type strains, were performed. Note the strains are congenic, but differ in whether the histones were present on TRP1 marked (H4) or LEU2 marked (H3) plasmids. Samples were analysed using Affymetrix S98 microarrays.

2007-09-21 | E-GEOD-4135 | biostudies-arrayexpress

Transcription profiling time series of S. cerevisiae wild type and upf1 mutant strains following transcription inhibition by thiolutin

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2007-07-12 | E-GEOD-3076 | biostudies-arrayexpress

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Project description:Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress. We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. Experiment Overall Design: The effects of oxidative stress induced by CHP on the transcriptional profile of S. cerevisiae was studied from a dynamical perspective. Yeast cultures were grown in controlled batch conditions, in 1 L fermentors. Three replicate cultures in mid-exponential phase were exposed to 0.19 mM CHP, while three non-treated cultures were used as controls. Samples were collected at t=0 (immediately before adding CHP) and at 3,6,12,20,40,70 and 120 min after adding the oxidant. Samples were processed for RNA extraction and profiled using Affymetrix Yeast Genome S98 arrays.

2008-06-15 | E-GEOD-7645 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae overexpressing transcriptional factors Kin28 and Pog1 suppresses the stress sensitivity caused by the rsp5 mutation

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2008-06-16 | E-GEOD-8729 | biostudies-arrayexpress

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2008-10-20 | E-GEOD-11236 | biostudies-arrayexpress

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2008-06-16 | E-GEOD-8326 | biostudies-arrayexpress

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2007-07-07 | E-GEOD-2407 | biostudies-arrayexpress

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