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RNA-seq of control and foxo3b MO zebrafish cells.

ABSTRACT: Gata1+ cells isolated from control and foxo3b MO zebrafish at 24 and 48hpf were subjected to RNA-seq and related analyses. Gata1-GFP zebrafish stran was utilized, embryos were injected with foxo3b MO and standard MO at one-cell stage. Gata1+ cells were selected by FACS at 24 and 48hpf and submitted to RNA-seq and related analyses.

ORGANISM(S): Danio rerio

SUBMITTER: yadong yang

PROVIDER: E-GEOD-66860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Knockdown of transcription factor forkhead box O3 (FOXO3) suppresses erythroid differentiation in human cells and zebrafish.

Wang Hai H Li Yanming Y Wang Sifeng S Zhang Qian Q Zheng Jiawen J Yang Yadong Y Qi Heyuan H Qu Hongzhu H Zhang Zhaojun Z Liu Feng F Fang Xiangdong X

Biochemical and biophysical research communications 20150403 4

Our previous study on the dynamic transcriptomes activated during human erythropoiesis suggested that transcription factor forkhead box O3 (FOXO3) possibly plays a role in erythroid differentiation. Functional studies in human cell line TF-1 indicated that FOXO3 knockdown repressed erythropoietin (EPO)-induced erythroid differentiation by activating promoter region of B-cell translocation gene 1 (BTG1), thereby regulating its expression. In zebrafish, injection of foxo3b-specific morpholinos (fo ...[more]

PMID: 25843800

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RNA-seq of control and foxo3b MO zebrafish cells.

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