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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes

ABSTRACT: Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Endosperm total and polysome-bound mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

ORGANISM(S): Zea mays

SUBMITTER: Weiwei Qi

PROVIDER: E-GEOD-67083 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Maize reas1 Mutant Stimulates Ribosome Use Efficiency and Triggers Distinct Transcriptional and Translational Responses.

Qi Weiwei W Zhu Jie J Wu Qiao Q Wang Qun Q Li Xia X Yao Dongsheng D Jin Ying Y Wang Gang G Wang Guifeng G Song Rentao R

Plant physiology 20151208 2

Ribosome biogenesis is a fundamental cellular process in all cells. Impaired ribosome biogenesis causes developmental defects; however, its molecular and cellular bases are not fully understood. We cloned a gene responsible for a maize (Zea mays) small seed mutant, dek* (for defective kernel), and found that it encodes Ribosome export associated1 (ZmReas1). Reas1 is an AAA-ATPase that controls 60S ribosome export from the nucleus to the cytoplasm after ribosome maturation. dek* is a weak mutant ...[more]

PMID: 26645456

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