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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse eGFP/CD3-double positive splenocytes to identify imprinted genes expressed in adult CD3+ splenocytes

ABSTRACT: Identification of imprinted genes expressed in adult CD3+ splenocytes Experiment Overall Design: For analysis of imprinted gene expression of uniparental-derived cells in adult recipients we chose eGFP/CD3-double positive splenocytes as a defined cell type, and first identified imprinted genes that are normally expressed in this cell type by microarray analysis of normal eGFP/CD3-double positive splenocytes from an eGFP transgenic B6129 mouse.

ORGANISM(S): Mus musculus

SUBMITTER: K John McLaughlin

PROVIDER: E-GEOD-6757 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hematopoietic reconstitution with androgenetic and gynogenetic stem cells.

Eckardt Sigrid S Leu N Adrian NA Bradley Heath L HL Kato Hiromi H Bunting Kevin D KD McLaughlin K John KJ

Genes & development 20070201 4

Parthenogenetic embryonic stem (ES) cells with two oocyte-derived genomes (uniparental) have been proposed as a source of autologous tissue for transplantation. The therapeutic applicability of any uniparental cell type is uncertain due to the consequences of genomic imprinting that in mammalian uniparental tissues causes unbalanced expression of imprinted genes. We transplanted uniparental fetal liver cells into lethally irradiated adult mice to test their capacity to replace adult hematopoieti ...[more]

PMID: 17322401

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