Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human Parp-1 deficient and wild type T cells following CD3 and CD3/CD28 activation


ABSTRACT: Primary T cells were isolated from spleen of Parp-1-/- and wild-type mice by magnetic depletion of non-T cells using a MACS Pan-T Cell isolation kit, according to the manufacturer´s instruction (Mintenyi Biotec, Bergisch Gladbach, Germany). Purity was assessed by flow cytometry analysis using antibodies against CD3, CD4 and CD8 and all preparations were more than 98% pure of T cells. The cells were activated with plate-bound anti-mouse CD3 (clone 145-2C11) (5 microg/ml) in the absence or the presence of anti-mouse CD28 (clone 37.51) (5microg/ml) both from BD PharMingen (San Diego, CA) and culture for 3.5 h in RPMI 1640 medium (BioWhittaker) supplemented with 10% FCS, 2mM L-glutamine, 5x10-5 M 2-mercaptoethanol (Sigma), 2.5 microg/ml fungizone, 100 IU/ml penicillin, and 10 microg/ml streptomycin.

ORGANISM(S): Mus musculus

SUBMITTER: Jose Yelamos 

PROVIDER: E-MEXP-1237 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional regulation by poly(ADP-ribose) polymerase-1 during T cell activation.

Saenz Luis L   Lozano Juan J JJ   Valdor Rut R   Baroja-Mazo Alberto A   Ramirez Pablo P   Parrilla Pascual P   Aparicio Pedro P   Sumoy Lauro L   Yélamos José J  

BMC genomics 20080416


<h4>Background</h4>Accumulating evidence suggests an important role for the enzyme poly(ADP-ribose) polymerase-1 (PARP-1) as an integral part of the gene expression regulatory machinery during development and in response to specific cellular signals. PARP-1 might modulate gene expression through its catalytic activity leading to poly(ADP-ribosyl)ation of nuclear proteins or by its physical association with relevant proteins. Recently, we have shown that PARP-1 is activated during T cell activati  ...[more]

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