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Detection of chromatin accessibility in WT and SRC-2-/- mice in liver

ABSTRACT: We futher characterized genome-wide chromatin accessibility of WT and SRC-2-/- mouse liver at CT10 through DNase-Seq. In addition,chromatin accessibility was significantly reduced in SRC-2-/- mouse liver compared to WT mice at CT10. DNase-Seq was carried out in WT and SRC-2-/- mice in liver at CT10 using two doses of DNaseI.

ORGANISM(S): Mus musculus

SUBMITTER: Bokai Zhu

PROVIDER: E-GEOD-67859 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude via REV-ERB Loading.

Zhu Bokai B Gates Leah A LA Stashi Erin E Dasgupta Subhamoy S Gonzales Naomi N Dean Adam A Dacso Clifford C CC York Brian B O'Malley Bert W BW

Molecular cell 20151121 5

A central mechanism for controlling circadian gene amplitude remains elusive. We present evidence for a "facilitated repression (FR)" model that functions as an amplitude rheostat for circadian gene oscillation. We demonstrate that ROR and/or BMAL1 promote global chromatin decondensation during the activation phase of the circadian cycle to actively facilitate REV-ERB loading for repression of circadian gene expression. Mechanistically, we found that SRC-2 dictates global circadian chromatin rem ...[more]

PMID: 26611104

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