Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide maps of BAF180 and REV-ERBα binding


ABSTRACT: We generated genome-wide cistromes of BAF180 subunit of the SWI-SNF chromatin remodeling complex in mouse liver at CT10 and CT22. In addition, we performed ChIP-Seq analysis on REV-ERBα in WT and SRC-2-/- mouse liver at CT10. We found circadian oscilation of BAF180 chromatin recruitment in mouse liver with peak recruitment at CT22 and nadir at CT10. Further,REV-ERBα chromatin recruitment was significantly reduced in SRC-2-/- mouse liver compared to WT mice at CT10. ChIP-Seq for BAF180 was performed in WT mice liver at CT10 and CT22 using two different antibodies. ChIP-Seq for REV-ERBα was performed in WT and SRC-2-/- mice in liver at CT10 with biological replicates.

ORGANISM(S): Mus musculus

SUBMITTER: Bokai Zhu 

PROVIDER: E-GEOD-67852 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude via REV-ERB Loading.

Zhu Bokai B   Gates Leah A LA   Stashi Erin E   Dasgupta Subhamoy S   Gonzales Naomi N   Dean Adam A   Dacso Clifford C CC   York Brian B   O'Malley Bert W BW  

Molecular cell 20151121 5


A central mechanism for controlling circadian gene amplitude remains elusive. We present evidence for a "facilitated repression (FR)" model that functions as an amplitude rheostat for circadian gene oscillation. We demonstrate that ROR and/or BMAL1 promote global chromatin decondensation during the activation phase of the circadian cycle to actively facilitate REV-ERB loading for repression of circadian gene expression. Mechanistically, we found that SRC-2 dictates global circadian chromatin rem  ...[more]

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