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Transcription profiling of Arabidopsis albino mutant DS 13-2198-1

ABSTRACT: The effect Ds insertion mutation in Ds13-2198-1 line on the gene expression profiles was investigated. The genes for photosynthesis and some transcriptional factors were upregulated while genes for metabolism were downregulated. Experiment Overall Design: To investigate the effect of an strong albino mutation, the genome wide gene expression profiles of plants were examined. Segregants with normal phenotype and albino phenotype obtained from the heterotalic parental line were grown on the same 1/2 MS plate for 3 weeks.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Takashi Hirayama 

PROVIDER: E-GEOD-6788 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Top-down phenomics of Arabidopsis thaliana: metabolic profiling by one- and two-dimensional nuclear magnetic resonance spectroscopy and transcriptome analysis of albino mutants.

Tian Chunjie C   Chikayama Eisuke E   Tsuboi Yuuri Y   Kuromori Takashi T   Shinozaki Kazuo K   Kikuchi Jun J   Hirayama Takashi T  

The Journal of biological chemistry 20070427 25

Elucidating the function of each gene in a genome is important for understanding the whole organism. We previously constructed 4000 disruptant mutants of Arabidopsis by insertion of Ds transposons. Here, we describe a top-down phenomics approach based on metabolic profiling that uses one-dimensional 1H and two-dimensional 1H,13C NMR analyses and transcriptome analysis of albino mutant lines of Arabidopsis. One-dimensional 1H NMR metabolic fingerprinting revealed global metabolic changes in the a  ...[more]

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