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Asthma III

ABSTRACT: Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data, contact Dr. Sally Wenzel (wenzelse@upmc.edu) Bronchoscopy with endobronchial epithelial brushing was performed as previously described (Chu et al., 2002; Zhao et al., 2011). Bronchial alveolar lavage fluids were spun down on 4000 g for 10 minutes. 0.5- 1X10^6 cells were stored in Trizol for RNA extraction. RNA in Trizol solution was extracted using the QIACube system (Qiagen, Valencia, CA). RNA quality was determined using the Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA), and only samples with an RIN higher than 7 used for microarray experiments.

ORGANISM(S): Homo sapiens

SUBMITTER: Jadranka Milosevic

PROVIDER: E-GEOD-67940 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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eQTL of bronchial epithelial cells and bronchial alveolar lavage deciphers GWAS-identified asthma genes.

Li X X Hastie A T AT Hawkins G A GA Moore W C WC Ampleford E J EJ Milosevic J J Li H H Busse W W WW Erzurum S C SC Kaminski N N Wenzel S E SE Meyers D A DA Bleecker E R ER

Allergy 20150724 10

<h4>Background</h4>Genome-wide association studies (GWASs) have identified various genes associated with asthma, yet, causal genes or single nucleotide polymorphisms (SNPs) remain elusive. We sought to dissect functional genes/SNPs for asthma by combining expression quantitative trait loci (eQTLs) and GWASs.<h4>Methods</h4>Cis-eQTL analyses of 34 asthma genes were performed in cells from human bronchial epithelial biopsy (BEC, n = 107) and from bronchial alveolar lavage (BAL, n = 94).<h4>Results ...[more]

PMID: 26119467

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