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Next Generation Sequencing of tomato WT, M82 and pro∆GRAS mutant treated with Gibberellin


ABSTRACT: The goal of this study was to search for DELLA-independent GA-regulated genes in tomato. RNA deep sequencing (RNA-seq) was performed to GA-treated M82 and pro∆GRAS mutant. Using a two fold increased or decreased cutoff we identified 81 GA-up regulated and 15 GA-down regulated genes, from which five genes were DELLA-independent. M82 and pro∆GRAS seedlings were treated with Paclobutrazol (10mg/l) once a day for three days followed by a single GA3 application (100 µM). Three hours after the GA treatment, young leaves were collected and RNA was extracted for Illumina HiSeq 2500 sequencing. A total of eight samples were analyzed, each treatment had two biological replicates.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: Gilgi Friedlander 

PROVIDER: E-GEOD-68018 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Uncovering DELLA-Independent Gibberellin Responses by Characterizing New Tomato procera Mutants.

Livne Sivan S   Lor Vai S VS   Nir Ido I   Eliaz Natanella N   Aharoni Asaph A   Olszewski Neil E NE   Eshed Yuval Y   Weiss David D  

The Plant cell 20150602 6


Gibberellin (GA) regulates plant development primarily by triggering the degradation/deactivation of the DELLA proteins. However, it remains unclear whether all GA responses are regulated by DELLAs. Tomato (Solanum lycopersicum) has a single DELLA gene named PROCERA (PRO), and its recessive pro allele exhibits constitutive GA activity but retains responsiveness to external GA. In the loss-of-function mutant pro(ΔGRAS), all examined GA developmental responses were considerably enhanced relative t  ...[more]

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