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Linker histone H1.2 establishes chromatin comapction and gene silencing through recognition of H3K27me3


ABSTRACT: Linker histone H1 is a protein component of chromatin and has been linked to chromatin compaction and global gene silencing.It has been sugegsted that H1 plays a significant role, regulating a relatively small number of genes. Here we show that H1.2- a variant of H1 subtype is recruited to chromatin region and is dependent on EZH2-mediated H3K27me3. Therefore a Gene expression array analysis was carried out with H1.2 as well as EZH2 knockout MCF7 cells to confirm the interlationship of H1.2 and EZH2 activity. Total RNA was isolated from MCF7 cells in which EZH2 and H1.2 genes have been depleted along with the wild type cells to study the differential regulation of various genes in each case.

ORGANISM(S): Homo sapiens

SUBMITTER: Vasu Punj 

PROVIDER: E-GEOD-68050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Linker histone H1.2 establishes chromatin compaction and gene silencing through recognition of H3K27me3.

Kim Jin-Man JM   Kim Kyunghwan K   Punj Vasu V   Liang Gangning G   Ulmer Tobias S TS   Lu Wange W   An Woojin W  

Scientific reports 20151119


Linker histone H1 is a protein component of chromatin and has been linked to higher-order chromatin compaction and global gene silencing. However, a growing body of evidence suggests that H1 plays a gene-specific role, regulating a relatively small number of genes. Here we show that H1.2, one of the H1 subtypes, is overexpressed in cancer cells and contributes to gene silencing. H1.2 gets recruited to distinct chromatin regions in a manner dependent on EZH2-mediated H3K27me3, and inhibits transc  ...[more]

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