Proteomics

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Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2


ABSTRACT: Post-translational modifications (PTMs) of histones have fundamental effects on chromatin structure and function. While the impact of PTMs on the function of core histones are increasingly well understood, this is much less the case for modifications of linker histone H1, which is at least in part due to a lack of proper tools. In this work, we establish the assembly of intact chromatosomes containing site-specifically ubiquitylated and acetylated linker histone H1.2 variants obtained by a combination of chemical biology approaches. We then use these complexes in a tailored affinity enrichment mass spectrometry workflow to identify and comprehensively characterize chromatosome-specific cellular interactomes and the impact of site-specific linker histone modifications on a proteome-wide scale. We validate and benchmark our approach by western-blotting and by confirming the involvement of chromatin-bound H1.2 in the recruitment of proteins involved in DNA double-strand break repair using an in vitro ligation assay. We relate our data to previous work and in particular compare it to data on modification-specific interaction partners of free H1. Taken together, our data supports the role of chromatin-bound H1 as a regulatory protein with distinct functions beyond DNA compaction and constitutes an important resource for future investigations of histone epigenetic modifications.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Philip Saumer  

LAB HEAD: Florian Stengel

PROVIDER: PXD040236 | Pride | 2023-11-02

REPOSITORIES: Pride

Dataset's files

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Action DRS
Q2203_saumerp_067.raw Raw
Q2203_saumerp_068.raw Raw
Q2203_saumerp_070.raw Raw
Q2203_saumerp_071.raw Raw
Q2203_saumerp_073.raw Raw
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Publications

Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2.

Saumer Philip P   Scheffner Martin M   Marx Andreas A   Stengel Florian F  

Nucleic acids research 20240101 1


Post-translational modifications (PTMs) of histones have fundamental effects on chromatin structure and function. While the impact of PTMs on the function of core histones are increasingly well understood, this is much less the case for modifications of linker histone H1, which is at least in part due to a lack of proper tools. In this work, we establish the assembly of intact chromatosomes containing site-specifically ubiquitylated and acetylated linker histone H1.2 variants obtained by a combi  ...[more]

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