Unknown,Transcriptomics,Genomics,Proteomics

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Identification of CLF-regulated transcripts in embryos at the mature-green stage by strand-specific RNA-sequencing in Arabidopsis


ABSTRACT: CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Our previous results suggested that CLF may play regulatory roles on seed size and lipid biosynthesis in embryos at the mature-green stages. Here we used strand specific RNA-sequencing to profile Arabidopsis transcriptomes obtained from isolated embryos at the mature-green stage of Col-0 and clf-28 plants. Our analysis identified a large number of CLF-regulatedd transcripts in developmenting embryos in Arabidopsis. Issolated embryos of WT and clf-28 with 2 biological replicates

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jun Liu 

PROVIDER: E-GEOD-68080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis.

Liu Jun J   Deng Shulin S   Deng Shulin S   Wang Huan H   Ye Jian J   Wu Hui-Wen HW   Sun Hai-Xi HX   Chua Nam-Hai NH  

Plant physiology 20160304 1


CURLY LEAF (CLF), a histone methyltransferase of Polycomb Repressive Complex 2 (PRC2) for trimethylation of histone H3 Lys 27 (H3K27me3), has been thought as a negative regulator controlling mainly postgermination growth in Arabidopsis (Arabidopsis thaliana). Approximately 14% to 29% of genic regions are decorated by H3K27me3 in the Arabidopsis genome; however, transcriptional repression activities of PRC2 on a majority of these regions remain unclear. Here, by analysis of transcriptome profiles  ...[more]

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