Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

37 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Systems analysis of uterine microenvironment in Treg depleted pregnant mice at 12 days after fertilization

ABSTRACT: Analysis of uterine microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchestrated the immune reponse triggered in presence of embryo. Total RNA obtained from uterine microenvironment tissues of Treg depleted pregnant mice (12 days after fertilization) compared to control tissues (uterus from PBS treated pregnant mice).

ORGANISM(S): Mus musculus

SUBMITTER: Djamel NEHAR-BELAID

PROVIDER: E-GEOD-68432 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Regulatory T Cells Orchestrate Similar Immune Evasion of Fetuses and Tumors in Mice.

Nehar-Belaid Djamel D Courau Tristan T Dérian Nicolas N Florez Laura L Ruocco Maria Grazia MG Klatzmann David D

Journal of immunology (Baltimore, Md. : 1950) 20151207 2

Embryos and tumors are both masses of dividing cells expressing foreign Ags, but they are not rejected by the immune system. We hypothesized that similar tolerogenic mechanisms prevent their rejection. Global comparison of fetal and tumor microenvironments through transcriptomics in mice revealed strikingly similar and dramatic decreases in expression of numerous immune-related pathways, including Ag presentation and T cell signaling. Unsupervised analyses highlighted the parallel kinetics and s ...[more]

PMID: 26643476

Similar Datasets

Systems analysis of uterine microenvironment in Treg depleted pregnant mice at 12 days after fertilization

2015-12-10 | GSE68432 | GEO

Systems analysis of uterine microenvironment 4, 6, 8, 10, 11 or 12 days after fertilization

Project description:Analysis of uterine microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchestrated the immune reponse triggered in presence of embryo Total RNA obtained from uterine microenvironment tissues (4, 6, 8, 10, 11 or 12 days after fertilization) compared to control tissues (uterus from non pregnant mice).

2015-12-10 | E-GEOD-68433 | biostudies-arrayexpress

Systems analysis of uterine microenvironment 4, 6, 8, 10, 11 or 12 days after fertilization

2015-12-10 | GSE68433 | GEO

Systems analysis of B16-WT, B16-shTGFb and B16-shVEGF tumor microenvironment 4 or 14 days after tumor inoculation

Project description:Analysis of B16 tumor microenvironments at gene expression level. The hypothesis tested in the present study was that Tregs orchastrated the immune reponse triggered in presence of tumors Total RNA obtained from tumor microenvironment tissues (at Day 4 and Day 14 after tumor inoculation) compared to control tissues.

2016-09-01 | E-GEOD-73384 | biostudies-arrayexpress

Transcriptome profile form endometrial biopsy samples of pregnant and non-pregnant cows

Project description:Purpose: characterize the uterine transcriptome profiles of pregnant (P) versus non-pregnant (NP) cows during early pregnancy and attempted to define a potential set of marker genes that can be valuable for predicting pregnancy outcome. Methods: beef cows were synchronized and artificially inseminated at detected estrus. Six days after AI, jugular blood samples and a biopsy from the uterine horn contralateral to the ovary containing the corpus luteum were collected. Based on pregnancy outcome on day 30, samples were retrospectively allocated to the following groups: Pregnant and Non-Pregnant. Both groups had similar plasma progesterone concentrations on D6. Uterine biopsies were submitted to RNA-Seq analysis in a Illumina HiScanSQ platform. Results: The 272,685,768 million filtered reads were mapped to the Bos taurus reference genome and 14,654 genes were analyzed for differential expression between groups. Transcriptome data showed that 216 genes are differently expressed when comparing NP versus P uterine tissue (Padj≤0.1). More specifically, 36 genes were up-regulated in P cows and 180 are up-regulated in NP cows. Conclusions: this study characterized a unique set of genes, expressed in the uterus on 6 days after insemination, that indicate a receptive state leading to pregnancy success. Furthermore, expression of such genes can be used as potential markers to efficiently predict pregnancy success. endometrial mRNA profiles of pregnant and non-pregnant cows were generated by deep sequencing using Illumina HiScanSQ platform BioProject PRJNA268916 SRA Study SRP05036

2015-04-09 | E-GEOD-65117 | biostudies-arrayexpress

Vagino-uterine microbiota in pregnant and non-pregnant beef cattle

Project description:Vagino-uterine microbiota in pregnant and non-pregnant beef cattle

| PRJNA976303 | ENA

Transcriptome profile form endometrial biopsy samples of pregnant and non-pregnant cows

2015-04-09 | GSE65117 | GEO

Transcription profiling of pig endometrium from pregnant and non-pregnant sows

Project description:In an attempt to unveil part of the molecular processes controlling porcine placentation we have investigated the pregnancy induced gene expression in the porcine endometrium at Day 14 after insemination using the Affymetrix GeneChip® Porcine Genome Array. Experiment Overall Design: At Day 14 after insemination, samples were obtained from the endometrium of pregnant sows and sows inseminated with inactivated semen for RNA extraction and hybridization on Affymetrix microarrays. The uteri were removed and each uterine horn was flushed with PBS containing 1% fetal calf serum, and subsequently opened longitudinally at the anti-mesometrial side. The sites of embryonic attachment were macroscopically visual in the endometrium on the mesometrial side in form of hyperemic zones. Samples of the endometrium (lamina epithelialis, lamina propria and tela submucosa, but not tunica muscularis) were isolated from proximal (the end close to the ovaries), intermedial, and distal (next to the corpus uteri)) sections of each uterine horn. Samples were taken from the endometrium of the non-pregnant animals at comparable locations.

2009-10-23 | E-GEOD-18641 | biostudies-arrayexpress

Gene expression profiling in the endometrium of pregnant mammals

Project description:A major challenge in biology is to determine how evolutionarily novel characters originate, however, mechanistic explanations for the origin of novelties are almost completely unknown. The evolution of mammalian pregnancy is an excellent system in which to study the origin of novelties because extant mammals preserve major stages in the transition from egg-laying to live-birth. To determine the molecular bases of this transition, we characterized the pregnant/gravid uterine transcriptome from tetrapods, including species in the three major mammalian lineages, and used ancestral transcriptome reconstruction to trace the evolutionary history of uterine gene expression. We show that thousands of genes evolved or lost uterine expression during the origins of mammalian pregnancy, including the loss of genes responsible for the mineralization of the eggshell in the Mammalian and Therian stem-lineages, and the recruitment of genes into uterine expression that mediate maternal-fetal communication and maternal immunotolerance of the fetal allograft in the Therian and Eutherian stem-lineages. Our results indicate that one of the defining mammalian novelties evolved through a step-wise process that dramatically changed uterine gene expression, generating a suite of evolutionary innovations that support prolonged gestations. We report genome-wide gene expression generated by mRNA-Seq from the pregnant endometrium of dog, armadillo, and the platypus.

2015-01-30 | E-GEOD-57714 | biostudies-arrayexpress

Systems analysis of B16 tumor microenvironment 4 or 14 days after tumor inoculation

Project description:Analysis of B16 tumor microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchastrated the immune reponse triggered in presence of tumors

2015-12-10 | GSE68434 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data